Gastric proton pump localization. Application of triphosphatase and monophosphatase techniques.

@article{Firth1981GastricPP,
  title={Gastric proton pump localization. Application of triphosphatase and monophosphatase techniques.},
  author={J. Anthony Firth and Geoffrey J. Stranks},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1981},
  volume={29 3},
  pages={344-50}
}
Potassium-dependent phosphatase activity can be demonstrated in unfixed frozen sections of mouse stomach using either adenosine triphosphate (ATP) or p-nitrophenyl phosphate (NPP) as substrate. In both cases the potassium-dependent reaction is confined to oxyntic cells, but with ATP, a strong, potassium-independent reaction occurs in the connective tissue of the lamina propria and elsewhere. In the NPP system potassium-independent reaction is very slight, and the oxyntic cell reaction shows… CONTINUE READING