Gas-phase purification enables accurate, large-scale, multiplexed proteome quantification with isobaric tagging

@inproceedings{Wenger2011GasphasePE,
  title={Gas-phase purification enables accurate, large-scale, multiplexed proteome quantification with isobaric tagging},
  author={Craig D. Wenger and Myung-ryul Lee and Alexander S. Hebert and G Mcalister and Douglas H Phanstiel and Michael S. Westphall and Joshua J. Coon},
  booktitle={Nature Methods},
  year={2011}
}
We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag–based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables… CONTINUE READING
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