Gas-permeable membranes and co-culture with fibroblasts enable high-density hepatocyte culture as multilayered liver tissues.

@article{Evenou2011GaspermeableMA,
  title={Gas-permeable membranes and co-culture with fibroblasts enable high-density hepatocyte culture as multilayered liver tissues.},
  author={Fanny Evenou and Morgan Hamon and Teruo Fujii and Shoji Takeuchi and Yasuyuki Sakai},
  journal={Biotechnology progress},
  year={2011},
  volume={27 4},
  pages={1146-53}
}
To engineer reliable in vitro liver tissue equivalents expressing differentiated hepatic functions at a high level and over a long period of time, it appears necessary to have liver cells organized into a three-dimensional (3D) multicellular structure closely resembling in vivo liver cytoarchitecture and promoting both homotypic and heterotypic cell-cell contacts. In addition, such high density 3D hepatocyte cultures should be adequately supplied with nutrients and particularly with oxygen… CONTINUE READING

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