Gas liquid chromatographic determination of the main urinary metabolite of diftalone, a new anti-inflammatory drug.

@article{Gallo1974GasLC,
  title={Gas liquid chromatographic determination of the main urinary metabolite of diftalone, a new anti-inflammatory drug.},
  author={G. G. Gallo and N. Rimorini and L. Zerilli and P. Radaelli},
  journal={Journal of chromatography},
  year={1974},
  volume={101 1},
  pages={
          163-8
        }
}
  • G. G. Gallo, N. Rimorini, +1 author P. Radaelli
  • Published 1974
  • Chemistry, Medicine
  • Journal of chromatography
  • Abstract The main metabolite of diftalone in humans is 7-hydroxydiftalone, which is present in urine as the β-glucuronide. Its determination is based on acid hydrolysis in the biological fluit itself to an isomeric carboxylicacid, extraction with ethyl acetate, esterification with diazomethane to the methyl ester and gas—liquid chromatographic analysis of this ester. The method is applicable to the urine of humans, dogs and rats with satisfactory precision and accuracy. The sensitivity is down… CONTINUE READING
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