Gas chromatographie determination of extracellular metabolites produced by baker’s yeast during glucose-induced acidification

Abstract

Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker’s yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5 % SE-52 in a programmed temperature interval of 80–220 °C (3 °C/min… (More)
DOI: 10.1007/BF02876610

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