Gas chromatographic-tandem mass spectrometric determination of anabolic steroids and their esters in hair. Application in doping control and meat quality control.

@article{Gaillard1999GasCM,
  title={Gas chromatographic-tandem mass spectrometric determination of anabolic steroids and their esters in hair. Application in doping control and meat quality control.},
  author={Yvan Gaillard and F Vayssette and Alain Balland and Gilbert P{\'e}pin},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1999},
  volume={735 2},
  pages={189-205}
}
We have developed a powerful and simple sensitive method for testing hair for anabolic steroids and their esters. A 100-mg amount of powdered hair was treated with methanol in an ultrasonic bath for extraction of esters, then alkaline digested with 1 M NaOH for an optimum recovery of other drugs. The two liquid preparations were subsequently extracted with ethyl acetate, pooled, then finally highly purified using a twin solid-phase extraction on amino and silica cartridges. The residue was… CONTINUE READING