Gas chromatographic mass spectrometric sequence determination of osteocalcin, a gamma-carboxyglutamic acid-containing protein from chicken bone.

@article{Carr1981GasCM,
  title={Gas chromatographic mass spectrometric sequence determination of osteocalcin, a gamma-carboxyglutamic acid-containing protein from chicken bone.},
  author={Steve A Carr and Peter V. Hauschka and Klaus Biemann},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 19},
  pages={9944-50}
}
The complete primary structure of osteocalcin, the gamma-carboxyglutamic acid (Gla)-containing calcium-binding protein isolated from chicken bone has been determined by gas chromatographic mass spectrometry. The method involves decarboxylation of the dry, intact protein in a low pressure atmosphere of DCl under conditions which quantitatively convert Gla into gamma, gamma-dideuteroglutamic acid residues without resulting in peptide bond cleavage. After partial enzymatic or acidic hydrolysis… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-7 of 7 extracted citations

Structure Determination of Natural Products by Mass Spectrometry.

Annual review of analytical chemistry • 2015
View 1 Excerpt

Matrix Gla protein in Xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research • 2001
View 1 Excerpt

Similar Papers

Loading similar papers…