Gas chromatographic-mass spectrometric identification of metabolites from alpha-pinene in human urine after occupational exposure to sawing fumes.

@article{Eriksson1996GasCS,
  title={Gas chromatographic-mass spectrometric identification of metabolites from alpha-pinene in human urine after occupational exposure to sawing fumes.},
  author={K{\aa}re A Eriksson and J O Levin},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1996},
  volume={677 1},
  pages={85-98}
}
Three metabolites from alpha-pinene (two diols and an alcohol with an aldehyde group) have been identified in human urine after occupational exposure to sawing fumes from pine. Urine was enzymatically hydrolyzed, cleaned up on a C18 micro-column and the metabolites were identified by GC-MS using electron impact (70 eV) and chemical ionization with ammonia or isobutane as the reagent gas. Analysis of underivatized metabolites was performed using a capillary column with a semi-polar phase and… CONTINUE READING