Gas chromatographic analysis of intact steryl esters in wild typeSaccharomyces cerevisiae and in an ester accumulating mutant
@article{Fenner2006GasCA, title={Gas chromatographic analysis of intact steryl esters in wild typeSaccharomyces cerevisiae and in an ester accumulating mutant}, author={G. P. Fenner and L. Parks}, journal={Lipids}, year={2006}, volume={24}, pages={625-629} }
The steryl ester faction from wild type and mutant strains of the yeastSaccharomyces cerevisiae was analyzed without saponification by a non-polar capillary gas chromatographic column. When expressed as μg ester/mg dry wt, the total ester fraction remained constant or declined slightly from log to stationary phase in the wild type. In the mutant the decrease was more dramatic. No individual ergosteryl ester species was dominant throughout the culture cycle in the wild type. A compound… CONTINUE READING
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