Gas Chromatography-Mass Spectrometry Measurement of 6β-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction

  title={Gas Chromatography-Mass Spectrometry Measurement of 6$\beta$-OH-Cortisol/Cortisol Ratio in Human Urine: A Specific Marker of Enzymatic Induction},
  author={Francesca Luceri and Stefania Fattori and Cristina Luceri and Meghan Zorn and Pier Francesco Mannaioni and Gianni Messeri},
  booktitle={Clinical Chemistry and Laboratory Medicine},
Abstract The urinary 6β-OH-cortisol/cortisol ratio is a specific, non-invasive marker for evaluating inductive or inhibitory effects on cytochrome P450 3A activity. We propose a new quantitative gas chromatographymass spectrometry with isotope dilution (GC-ID-MS) method for the simultaneous determination of urinary free cortisol (UFC) and 6β-OH-cortisol (6β-OHC). The method utilizes the following: (a) addition of internal standard (2H2 cortisol) to 1 ml of urine; (b) loading on to an Extrelut… 

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