Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.

@article{Shortle1982GapMM,
  title={Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.},
  author={David Shortle and Paula Grisafi and Stephen J Benkovic and David Botstein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 5},
  pages={1588-92}
}
Short single-stranded gaps can be constructed by limited exonuclease action at single-stranded breaks (nicks) placed at predetermined sites on closed circular DNA molecules. As efficient primer-templates for DNA polymerase, single-stranded gaps can be repaired in vitro to regenerate an intact DNA duplex. In this report two in vitro reaction schemes are described that produce a high frequency of errors during repair ("misrepair") of gaps and thereby allow the efficient recovery of mutations… CONTINUE READING

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