Ganglioside GM3 sialidase activity in fibroblasts of normal individuals and of patients with sialidosis and mucolipidosis IV. Subcellular distribution and and some properties.

Abstract

Sensitive assays for the determination of the ganglioside sialidase activity of fibroblast homogenates were established using ganglioside GM3, 3H-labelled in the sphingosine moiety, as a substrate. Ganglioside GM3 sialidase activity was greatly stimulated by the presence of the non-ionic detergent Triton X-100 and was further enhanced by salts such as NaCl… (More)

Topics

Cite this paper

@article{Lieser1989GangliosideGS, title={Ganglioside GM3 sialidase activity in fibroblasts of normal individuals and of patients with sialidosis and mucolipidosis IV. Subcellular distribution and and some properties.}, author={Manfred Lieser and Etti Harms and Heidrun Kern and Gideon Bach and Michael Cantz}, journal={The Biochemical journal}, year={1989}, volume={260 1}, pages={69-74} }