Gamma-glutamyltranspeptidase of Helicobacter pylori induces mitochondria-mediated apoptosis in AGS cells.

@article{Kim2007GammaglutamyltranspeptidaseOH,
  title={Gamma-glutamyltranspeptidase of Helicobacter pylori induces mitochondria-mediated apoptosis in AGS cells.},
  author={Kyung Mi Kim and Seung-Gyu Lee and Min-gyu Park and Jea-Young Song and H Kang and Woo-Kon Lee and M K Cho and Kwang Ho Rhee and Hee Shang Youn and Seung Chul Baik},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={355 2},
  pages={562-7}
}
Gamma-glutamyltranspeptidase (GGT) is a novel protein involved in the induction of Helicobacter pylori-mediated apoptosis; however, the signal pathway involved in GGT-induced apoptosis remains unclear. Using DNA recombination techniques, ggt was cloned into pET117b and transformed into Escherichia coli. Recombinant GGT was purified using nickel-affinity resin and was digested by thrombin. Recombinant GGT induced apoptosis in AGS cells in a time-dependent manner, which was confirmed by TUNEL… CONTINUE READING