Galactosylononitol and stachyose synthesis in seeds of adzuki bean. Purification and characterization of stachyose synthase.

  title={Galactosylononitol and stachyose synthesis in seeds of adzuki bean. Purification and characterization of stachyose synthase.},
  author={Thomas Peterbauer and Andreas Richter},
  journal={Plant physiology},
  volume={117 1},
Stachyose synthase (STS) (EC was purified to homogeneity from mature seeds of adzuki bean (Vigna angularis). Electrophoresis under denaturing conditions revealed a single polypeptide of 90 kD. Size-exclusion chromatography of the purified enzyme yielded two activity peaks with apparent molecular masses of 110 and 283 kD. By isoelectric focusing and chromatofocusing the protein was separated into several active forms with isoelectric point values between pH 4. 7 and 5.0. Purified STS… 

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