Galactosamine-induced apoptosis in the primary mouse hepatocyte cultures.

  title={Galactosamine-induced apoptosis in the primary mouse hepatocyte cultures.},
  author={Shunichi O. Tsutsui and Ken-ichi Hirasawa and Makio Takeda and Shin-ichi Itagaki and Seiichi Kawamura and Kazuhiko Maeda and Takashi Mikami and Kunio Doi},
  journal={Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie},
  volume={49 3-4},
Galactosamine (GalN)-induced apoptosis was investigated in cultured hepatocytes from mice. The percentage of fragmented DNA measured by the ELISA method increased in a concentration-dependent manner from the very early stage, i.e. 0.5 hrs, after GalN-exposure. In addition, a ladder-like fragmentation pattern by agarose gel electrophoresis appeared first at 3 hr-exposure to 20 mM GalN, at 6 hr-exposure to 10 mM GalN and at 12 hr-exposure to 5 mM GalN, respectively. On the other hand… CONTINUE READING