Gal4-based Enhancer-Trapping in the Malaria Mosquito Anopheles stephensi

  title={Gal4-based Enhancer-Trapping in the Malaria Mosquito Anopheles stephensi},
  author={David A. O’Brochta and Kristina L. Pilitt and Robert A. Harrell and Channa U Aluvihare and Robert T. Alford},
Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human malaria vector, Anopheles stephensi. The system consists of six transgenic lines of Anopheles stephensi, each with a single piggyBac-Gal4 element in a unique genomic location; six lines with a single piggyBac-UAStdTomato element; and two lines, each with a… CONTINUE READING