Gain-of-function phenotypes of chemically synthetic CLAVATA3/ESR-related (CLE) peptides in Arabidopsis thaliana and Oryza sativa.

@article{Kinoshita2007GainoffunctionPO,
  title={Gain-of-function phenotypes of chemically synthetic CLAVATA3/ESR-related (CLE) peptides in Arabidopsis thaliana and Oryza sativa.},
  author={Atsuko Kinoshita and Yasukazu Nakamura and Erika Sasaki and Junko Kyozuka and Hiroo Fukuda and Shinichiro Sawa},
  journal={Plant \& cell physiology},
  year={2007},
  volume={48 12},
  pages={
          1821-5
        }
}
Using 26 chemically synthetic CLAVATA3/ESR (CLE) peptides, which correspond to the predicted products of the 31 Arabidopsis CLE genes, we investigated the CLE peptide function in Arabidopsis and rice. Treatment with some CLE peptides inhibited root elongation in rice as well as in Arabidopsis. It also reduced the size of the shoot apical meristem in Arabidopsis but not in rice. Database searches revealed 47 putative CLE genes in the rice genome and multiple CLE domains in some CLE genes… 
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143 Citations

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A large family of genes that share homology with CLE domain in Arabidopsis and rice
TLDR
Results indicate conserved and/or diverse CLE functions in each plant species.
Structural and functional diversity of CLAVATA3/ESR (CLE)-like genes from the potato cyst nematode Globodera rostochiensis.
TLDR
The results reveal that G. rostochiensis CLE proteins with either single or multiple CLE motifs function similarly to plant CLE proteins and that CLE signaling components are conserved in both Arabidopsis and potato roots.
Diverse and conserved roles of CLE peptides.
CLE genes in plant development
  • T. Strabala
  • Biology, Environmental Science
    Plant signaling & behavior
  • 2008
TLDR
The gain-of-function studies demonstrated that all of the 18 CLE genes caused pleiotropic and often opposing phenotypes, including various combinations of increased root and rosette growth, root stunting, dwarfing, shoot apical meristem arrest, asymmetric leaf development, and “shrub-like” phenotypes.
Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.
TLDR
This work represents the first report on the functional analysis of CLE genes in a tree species and constitutes a basis for further study of the CLE peptide signalling pathway in tree development.
CLE signaling systems during plant development and nematode infection.
TLDR
The latest research uncovering the diverse functions and activities ofCLE peptides in plants; especially during shoot, root and vascular development is discussed and the important role of CLE peptides during infection by phytoparasitic nematodes is discussed.
The Receptor-Like Kinase SOL2 Mediates CLE Signaling in Arabidopsis
TLDR
It is suggested that SOL2, together with CLV2, plays an important role in the regulation of root meristem development through the CLE signaling pathway.
The Function of the CLE Peptides in Plant Development and Plant-Microbe Interactions
TLDR
Understanding the molecular properties of CLE peptides may provide insight into plant cell-cell communication, and therefore also into plant-microbe interactions, through the CLE-receptor kinase-WOX signaling node.
A CLE-WOX signalling module regulates root meristem maintenance and vascular tissue development in rice.
TLDR
It is shown that exogenous application of a synthetic dodeca-amino acid peptide corresponding to the CLE motif of the rice FON2-LIKE CLE PROTEIN2 (FCP2p) protein or overexpression of FCP2 terminates root apical meristem activity and impairs late metaxylem formation, suggesting that in rice RAM maintenance and late metAXylem development are probably controlled by the mutual regulation between FCP1 and QHB.
Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells
TLDR
It is highlighted that vascular patterning is a process controlled in time and space by different CLE peptides in conjunction with hormonal signaling.
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References

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Gain-of-Function Phenotypes of Many CLAVATA3/ESR Genes, Including Four New Family Members, Correlate with Tandem Variations in the Conserved CLAVATA3/ESR Domain1[W]
TLDR
The results indicate the capacity for functional redundancy of many of the CLE ligands and overexpression phenotypes of various CLE family members suggest roles in organ size regulation, apical dominance, and root growth.
CLV3/ESR-related (CLE) peptides as intercellular signaling molecules in plants.
TLDR
Two natural CLAVATA3 (CLV3)/ESR-related (CLE) peptides are discovered: tracheary elements differentiation inhibitory factor (TDIF) and CLV3, which are dodecapeptides with two hydroxyproline residues that regulate vascular development and meristem formation, respectively, and are used to predict the chemical form of CLE gene products.
The ArabidopsisCLV3-like (CLE) genes are expressed in diverse tissues and encode secreted proteins
TLDR
The results suggest that the Arabidopsis CLE proteins, like CLV3, may function as secreted signaling molecules that act in diverse pathways during growth and development.
Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems.
TLDR
Genetic analysis indicates that CLV1, which encodes a receptor kinase, acts with CLV3 to control the balance between meristem cell proliferation and differentiation in Arabidopsis thaliana plants with loss-of-function mutations in the CLAVATA genes.
Dodeca-CLE Peptides as Suppressors of Plant Stem Cell Differentiation
TLDR
An application, corresponding to all 26 Arabidopsis CLE protein family members, of synthetic dodecapeptides reveals two counteracting signaling pathways involved in stem cell fate.
Evidence for Functional Conservation, Sufficiency, and Proteolytic Processing of the CLAVATA3 CLE Domain1[W][OA]
TLDR
It is proposed that CLV3 and other CLEs are C-terminally processed to generate an active CLE peptide and detected proteolytic activity in extracts from cauliflower that process both CLv3 and CLE1 at their C termini.
A Plant Peptide Encoded by CLV3 Identified by in Situ MALDI-TOF MS Analysis
The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell–specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization–time-of-flight mass…
Conservation and diversification of meristem maintenance mechanism in Oryza sativa: Function of the FLORAL ORGAN NUMBER2 gene.
TLDR
Results clearly indicate that the FON1-FON2 system in rice corresponds to the CLV signaling system in Arabidopsis and suggest that the negative regulation of stem cell identity by these systems may be principally conserved in a wide range of plants within the Angiosperms.
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TLDR
The isolation and characterization of three floral organ number4 (fon4) alleles and the cloning of the FON4 gene revealed it encodes a small putatively secreted protein, which is the putative ortholog of the Arabidopsis (Arabidopsis thaliana) CLAVATA3 (CLV3) gene.
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