Gain-of-function Mutations Reveal Expanded Intermediate States and a Sequential Action of Two Gates in MscL

@article{Anishkin2005GainoffunctionMR,
  title={Gain-of-function Mutations Reveal Expanded Intermediate States and a Sequential Action of Two Gates in MscL},
  author={Andriy Anishkin and C Chiang and Sergei Sukharev},
  journal={The Journal of General Physiology},
  year={2005},
  volume={125},
  pages={155 - 170}
}
The tension-driven gating transition in the large mechanosensitive channel MscL proceeds through detectable states of intermediate conductance. Gain-of-function (GOF) mutants with polar or charged substitutions in the main hydrophobic gate display altered patterns of subconducting states, providing valuable information about gating intermediates. Here we present thermodynamic analysis of several GOF mutants to clarify the nature and position of low-conducting conformations in the transition… CONTINUE READING

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