GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

@article{Jefferson1987GUSFB,
  title={GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.},
  author={Richard Anthony Jefferson and Thomas Anthony Kavanagh and Michael W. Bevan},
  journal={The EMBO journal},
  year={1987},
  volume={6 13},
  pages={
          3901-7
        }
}
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We have constructed gene fusions using the cauliflower mosaic virus (CaMV) 35S promoter or the promoter from a gene encoding the small subunit of ribulose bisphosphate carboxylase (rbcS) to direct the expression of beta… CONTINUE READING
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