GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles.

@article{Lanoix1999GTPHB,
  title={GTP hydrolysis by arf-1 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles.},
  author={Joel Lanoix and Joke Ouwendijk and Colin C Lin and Annika Stark and Harold D. Love and Jens Ostermann and Tommy Nilsson},
  journal={The EMBO journal},
  year={1999},
  volume={18 18},
  pages={4935-48}
}
Upon addition of GTPgammaS to in vitro budding reactions, COP I vesicles form but retain their coat, making them easy to isolate and analyze. We have developed an in vitro budding assay that reconstitutes the formation of COP I-derived vesicles under conditions where GTP hydrolysis can occur. Once formed, vesicles are uncoated and appear functional as they fuse readily with acceptor membranes. Electron microscopy shows a homogeneous population of uncoated vesicles that contain the medial/trans… CONTINUE READING
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