GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission

@article{Roux2006GTPdependentTO,
  title={GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission},
  author={Aur{\'e}lien Roux and Katherine E. Uyhazi and Adam Frost and Pietro De Camilli},
  journal={Nature},
  year={2006},
  volume={441},
  pages={528-531}
}
Dynamin, a crucial factor in endocytosis, is a member of a family of GTPases that participates in membrane fission. It was initially proposed to act as a machine that constricts and cuts the neck of nascent vesicles in a GTP-hydrolysis-dependent reaction, but subsequent studies suggested alternative models. Here we monitored the effect of nucleotides on dynamin-coated lipid tubules in real time. Addition of GTP, but not of GDP or GTP-γS, resulted in twisting of the tubules and supercoiling… 
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