GTP-binding proteins in brain and neutrophil are tethered to the plasma membrane via their amino termini.

@article{Eide1987GTPbindingPI,
  title={GTP-binding proteins in brain and neutrophil are tethered to the plasma membrane via their amino termini.},
  author={Bj\orn Eide and Peter Gierschik and Graeme Milligan and Ian Mullaney and Cecilia G. Unson and Paul Goldsmith and Allen M. Spiegel},
  journal={Biochemical and biophysical research communications},
  year={1987},
  volume={148 3},
  pages={1398-405}
}
Using specific antisera raised against synthetic peptides, we find that three distinct GTP-binding protein alpha subunits remain bound to the plasma membrane even after activation with nonhydrolyzable GTP analog. Trypsin cleaves each alpha subunit at a site near the amino-terminus, and quantitatively releases the large fragment (comprising all but an amino-terminal 2 kDa piece) from the membrane. Our results indicate that alpha subunits are essentially cytoplasmic proteins tethered to the inner… CONTINUE READING
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