GMP-manufactured density gradient media for optimized mesenchymal stromal/stem cell isolation and expansion.

  title={GMP-manufactured density gradient media for optimized mesenchymal stromal/stem cell isolation and expansion.},
  author={Giulia Grisendi and Cecilia Anner{\'e}n and Luigi Cafarelli and Rita Sternieri and Elena Veronesi and Gian Luca Cervo and Stefano Luminari and Michela Maur and Antonio Frassoldati and Giovanni Palazzi and Satoru Otsuru and Franco Bambi and Paolo Paolucci and Conte Pierfranco and Edwin Horwitz and Massimo Dominici},
  volume={12 4},
BACKGROUND AIMS Bone marrow (BM) mesenchymal stromal/stem cells (MSC) are therapeutic tools in regenerative medicine and oncology. MSC isolation is often performed starting from a separation step based on research-grade 1.077 g/mL density gradient media (DGM). However, MSC clinical application should require the introduction of good manufacturing practice (GMP) reagents. We took advantage of two novel GMP DGM with densities of 1.077 and 1.073 g/mL (Ficoll-Paque PREMIUM and Ficoll-Paque PREMIUM… CONTINUE READING


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