GM2-ganglioside metabolism in cultured human skin fibroblasts: unambiguous diagnosis of GM2-gangliosidosis.

@article{Raghavan1985GM2gangliosideMI,
  title={GM2-ganglioside metabolism in cultured human skin fibroblasts: unambiguous diagnosis of GM2-gangliosidosis.},
  author={Srinivasa S. Raghavan and Allan Krusell and Timothy A. Lyerla and Eric G. Bremer and Edwin H. Kolodny},
  journal={Biochimica et biophysica acta},
  year={1985},
  volume={834 2},
  pages={238-48}
}
The metabolism of GM2-ganglioside was studied in situ using cultured skin fibroblasts from normal individuals and patients with different forms of GM2-gangliosidosis. [3H]Sphingosine-labeled GM2 was provided in the culture medium to confluent cells in 6-cm petri dishes. After 10 days, the cells were washed free of radioactivity and harvested by trypsinization. The cellular lipids were extracted and analyzed for radioactivity in GM2 and its metabolic products. In fibroblasts from healthy… CONTINUE READING