GFT NMR experiments for polypeptide backbone and 13Cbeta chemical shift assignment.

@article{Kim2004GFTNE,
  title={GFT NMR experiments for polypeptide backbone and 13Cbeta chemical shift assignment.},
  author={Seho Kim and Thomas Szyperski},
  journal={Journal of biomolecular NMR},
  year={2004},
  volume={28 2},
  pages={117-30}
}
(4,3)D, (5,3)D and (5,2)D GFT triple resonance NMR experiments are presented for polypeptide backbone and (13)C(beta) resonance assignment of (15)N/(13)C labeled proteins. The joint sampling of m = 2, 3 or 4 indirect chemical shift evolution periods of 4D and 5D NMR experiments yields the measurement of 2(m) - 1 linear combinations of shifts. To obtain sequential assignments, these are matched in corresponding experiments delineating either intra or interresidue correlations. Hence, an… CONTINUE READING

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