GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage

@article{Cai2006GFPEA,
  title={GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage},
  author={Xiao-dong Cai and Xin Yong Liu and Wen-wu Guo},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2006},
  volume={87},
  pages={245-253}
}
Somatic hybridization was performed via electrofusion between embryogenic suspension-derived protoplasts of transgenic green fluorescent protein (GFP) Satsuma mandarin (Citrus unshiu Marc. cv. Guoqing No. 1) (G1) callus and mesophyll protoplasts of calamondin (Citrus microcarpa Bunge), and three embryoids expressing GFP under UV light were obtained after 60 days of culture. The three embryoids were considered not as diploid cybrids but true allotetraploid somatic hybrids, as it was based on: (1… CONTINUE READING

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