GCP 5 and GCP 6 : Two New Members of the Human-Tubulin Complex

  title={GCP 5 and GCP 6 : Two New Members of the Human-Tubulin Complex},
  author={Steven M. Murphy and Andrea M. Preble and U. K. Patel and Kathy L. O’Connell and D. Prabha Dias and Michelle Kok Moritz and David Agard and John T. Stults and Tim Stearns},
The -tubulin complex is a large multiprotein complex that is required for microtubule nucleation at the centrosome. Here we report the purification and characterization of the human -tubulin complex and the identification of its subunits. The human -tubulin complex is a ring of 25 nm, has a subunit structure similar to that reported for -tubulin complexes from other species, and is able to nucleate microtubule polymerization in vitro. Mass spectrometry analysis of the human -tubulin complex… CONTINUE READING
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