GC-IMS headspace analyses allow early recognition of bacterial growth and rapid pathogen differentiation in standard blood cultures

  title={GC-IMS headspace analyses allow early recognition of bacterial growth and rapid pathogen differentiation in standard blood cultures},
  author={Carolin Drees and Wolfgang Vautz and Sascha Liedtke and Christophe Rosin and Kirsten Althoff and Martin Lippmann and Stefan Zimmermann and Tobias J. Legler and Duygu Yildiz and Thorsten Perl and Nils Kunze-Szikszay},
  journal={Applied Microbiology and Biotechnology},
  pages={9091 - 9101}
Outcome of patients with blood stream infections (BSI) depends on the rapid initiation of adequate antibiotic therapy, which relies on the fast and reliable identification of the underlying pathogen. Blood cultures (BC) using CO2-sensitive colorimetric indicators and subsequent microbiological culturing are the diagnostic gold standard but turnaround times range between 24 and 48 h. The detection of volatile organic compounds of microbial origin (mVOC) has been described as a feasible method… 
Blood Culture Headspace Gas Analysis Enables Early Detection of Escherichia coli Bacteremia in an Animal Model of Sepsis
The results are promising and warrant further evaluation in studies with an extended microbial panel and indications concerning its transferability to human samples.
Rapid in vitro differentiation of bacteria by ion mobility spectrometry
MCC-IMS is able to differentiate the tested bacterial species, even the non-resistant and their corresponding resistant strains, based on VOC patterns after 90 min of cultivation, which is very promising.
Use of GC-IMS for detection of volatile organic compounds to identify mixed bacterial culture medium
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Identification of volatile compounds from bacteria by spectrometric methods in medicine diagnostic and other areas: current state and perspectives
A narrative review introduces the application of spectrometric analytical methods for the purpose of detecting VOCs of bacterial origin and their clinical use for diagnosing different infectious conditions over the last decade.
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Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile
Current available methods for the clinical diagnosis of urinary tract infection rely on a urine dipstick test or culturing of pathogens, but there is a growing need for alternative diagnostic tools and there is great potential for these technologies to gain wide-spread and routine use in clinical settings, since the analysis can be automated, and test results can be available within minutes after sample collection.
Pilot Testing of the “Turbidimeter”, a Simple, Universal Reader Intended to Complement and Enhance Bacterial Growth Detection in Manual Blood Culture Systems in Low-Resource Settings
A simple, universal reader prototype that detects bacterial growth in blood culture bottles that evaluates bacterial growth, based on the turbidity of the broth and the color change of the colorimetric CO2 indicator in commercially available bloodculture bottles, performs well and is identified for improvements.
On-Line Coupling of Chip-Electrochromatography and Ion Mobility Spectrometry.
This approach combines the separation power of two electrokinetically driven separation techniques, the first in liquid phase and the second in gas phase, with a label-free detection of the analytes, and has great potential as a portable analytical system for field analysis of complex mixtures.
Coupling Droplet Microfluidics with Ion Mobility Spectrometry for Monitoring Chemical Conversions at Nanoliter Scale.
The successful coupling of droplet microfluidics with a custom-built high-resolution drift tube IMS system for monitoring chemical reactions in nL-sized droplets in an oil phase and the chemical-specific monitoring of a biochemical transformation catalyzed by a few hundred yeast cells, at single droplet level are demonstrated.
Miniaturized Drift Tube Ion Mobility Spectrometer with Ultra-Fast Polarity Switching.
This work focuses on applying ultra-fast polarity switching to an existing IMS, and the techniques shown here may be applied to other IMS implementations for different applications.


Detection and validation of volatile metabolic patterns over different strains of two human pathogenic bacteria during their growth in a complex medium using multi-capillary column-ion mobility spectrometry (MCC-IMS)
The results show that differentiation of human pathogenic bacteria by headspace analyses using MCC-IMS technology is best possible during the late phases of bacterial growth and show that VOC patterns of a bacterial strain can be transferred to other strains of the same species.
Ion mobility spectrometry for microbial volatile organic compounds: a new identification tool for human pathogenic bacteria
In a hospital routine, the innovative method multi-capillary column–ion mobility spectrometry (MCC-IMS) could enable the identification of pathogens already after 24 h with the consequence that a specific therapy could be initiated significantly earlier.
Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis.
  • H. WesthG. Lisby A. Hoeft
  • Medicine, Biology
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2009
A novel multiplex PCR test (SeptiFast (alpha version) that allows identification of 20 bacterial and fungal species directly from blood was used, comparatively with BC, in a multicentre trial of patients with suspected bacterial or fungal sepsis, to assess whether the use of SeptiFast is of significant advantage in the detection of bloodstream pathogens.
Headspace volatile organic compounds from bacteria implicated in ventilator-associated pneumonia analysed by TD-GC/MS.
Media components (substrates) were investigated to identify those that are essential for indole and cyclopentanone production, with potential implications for understanding microbial metabolism in the lung.
On-Line Monitoring of Microbial Volatile Metabolites by Proton Transfer Reaction-Mass Spectrometry
The findings strongly indicate that the temporal evolution of V OC emissions during growth must be considered if characterization or differentiation based on microbial VOC emissions is attempted, and may help to establish the analysis of VOCs by on-line PTR-MS as a routine method in microbiology and as a tool for monitoring environmental and biotechnological processes.
Detection of characteristic metabolites of Aspergillus fumigatus and Candida species using ion mobility spectrometry – metabolic profiling by volatile organic compounds
Volatile metabolites of Aspergillus fumigatus and Candida species can be detected by gas chromatography/mass spectrometry (GC/MS). A multi‐capillary column – ion mobility spectrometer (MCC‐IMS) was
Blood Culture Turnaround Time in U.S. Acute Care Hospitals and Implications for Laboratory Process Optimization
The differences in turnaround times and the times from when specimens were collected and the results were reported in the 24-h spectrum are compared to suggest laboratory automation and work flow optimization may play important roles in reducing the microbiology result turnaround time.
Characteristics of volatile organic compounds produced from five pathogenic bacteria by headspace‐solid phase micro‐extraction/gas chromatography‐mass spectrometry
The characteristics of volatiles from the five foodborne pathogens could lay a fundamental basis for further research into pathogen contamination control by detecting volatile signatures of pathogens.
Rapid detection of health-care-associated bloodstream infection in critical care using multipathogen real-time polymerase chain reaction technology: a diagnostic accuracy study and systematic review.
A systematic review of Phase III diagnostic accuracy studies of SeptiFast reveals uncertainty about its likely clinical utility based on widespread evidence of deficiencies in study design and reporting with a high risk of bias, and indicates that BC has a low sensitivity, questioning its relevance as a reference test in this setting.