GABA synthesis mediated by γ-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and α-ketoglutarate decarboxylase genes.

  title={GABA synthesis mediated by $\gamma$-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and $\alpha$-ketoglutarate decarboxylase genes.},
  author={Simab Kanwal and Aran Incharoensakdi},
  journal={Plant science : an international journal of experimental plant biology},
  • S. Kanwal, A. Incharoensakdi
  • Published 1 January 2020
  • Biology, Chemistry
  • Plant science : an international journal of experimental plant biology

GABA as a signalling molecule: Possible mechanism for its enhanced commercial production by cyanobacteria

The present review highlights the metabolic pathways of GABA production in cyanobacteria particularly the possible ways to enhance the endogenous GABA pool and its extraction in cost effective way to meet the rising demand due to its diverse physiological functions on human health.

Recent Advances in the Photoautotrophic Metabolism of Cyanobacteria: Biotechnological Implications

New important discoveries on the plasticity of the photoautotrophic metabolism of cyanobacteria are summarized, emphasizing the coordinated partitioning of carbon and nitrogen towards growth or compound storage, and the importance of these processes for biotechnological perspectives.



The role of GAD pathway for regulation of GABA accumulation and C/N balance in Synechocystis sp. PCC6803

Overall results suggested that the optimization of carbon and nitrogen sources was effective in enhancing the GABA accumulation in Synechocystis overexpressing the GAD and lacking the functional KGD pathway.

Disruption of cyanobacterial γ-aminobutyric acid shunt pathway reduces metabolites levels in tricarboxylic acid cycle, but enhances pyruvate and poly(3-hydroxybutyrate) accumulation

Disruption of the GABA shunt pathway reduced the TCA cycle metabolites levels, but positively enhanced the bioaccumulation of pyruvate and PHB.

The γ‐aminobutyric acid shunt contributes to closing the tricarboxylic acid cycle in Synechocystis sp. PCC 6803

C‐stable isotope analysis indicated that the γ‐aminobutyrate shunt is a major contributor to flux from 2‐oxoglutarate and glutamate to succinate in Synechocystis sp.

Natural and Synthetic Variants of the Tricarboxylic Acid Cycle in Cyanobacteria: Introduction of the GABA Shunt into Synechococcus sp. PCC 7002

It is shown that N-acetylornithine aminotransferase (ArgD) can function as a GABA aminotsferase and that, together with glutamate decarboxylase (GadA), it can complete a functional GABA shunt.

GABA Accumulation in Response to Different Nitrogenous Compounds in Unicellular Cyanobacterium Synechocystis sp. PCC 6803

Overall results suggested that optimization of nitrogen source and nitrogenous compounds supplementation was effective for the enhancement of GABA accumulation in Synechocystis.

Arabidopsis aldehyde dehydrogenase 10 family members confer salt tolerance through putrescine-derived 4-aminobutyrate (GABA) production

Root growth of single loss-of-function mutants was more sensitive to salinity than wild-type plants, and this was accompanied by reduced GABA accumulation.

4-Aminobutyrate (GABA): a metabolite and signal with practical significance

The origin of 4-aminobutyrate (GABA) from glutamate and polyamines, and its subsequent catabolism to succinic semialdehyde and either succinate or 4-hydroxybutyrate is discussed, demonstrating that stress-induced GABA metabolism is strongly linked with respiration.

Regulation of Γ‐aminobutyric acid synthesis in situ by glutamate availability

Evidence is provided for increased flux of carbon through the Gaba shunt when the availability of Glu as a substrate for Glu decarboxylase was enhanced, and secondary effects that can lead to an inhibition of photosynthesis were minimized.