G-protein regulation of outwardly rectified epithelial chloride channels incorporated into planar bilayer membranes.

@article{Ismailov1996GproteinRO,
  title={G-protein regulation of outwardly rectified epithelial chloride channels incorporated into planar bilayer membranes.},
  author={Iskander I. Ismailov and Biljana Jovov and Catherine M. Fuller and Bakhrom K. Berdiev and Deborah A Keeton and Dale J. Benos},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 9},
  pages={
          4776-80
        }
}
Experiments were designed to test if immunopurified outwardly rectified chloride channels (ORCCs) and the cystic fibrosis transmembrane conductance regulator (CFTR) incorporated into planar lipid bilayers are regulated by G-proteins. pertussis toxin (PTX) (100 ng/ml) + NAD (1 mM) + ATP (1 mM) treatment of ORCC and CFTR in bilayers resulted in a 2-fold increase in single channel open probability (Po) of ORCC but not of CFTR. Neither PTX, NAD, nor ATP alone affected the biophysical properties of… CONTINUE READING
BETA