G-protein-effector coupling: a real-time light-scattering assay for transducin-phosphodiesterase interaction.

@article{Heck1993GproteineffectorCA,
  title={G-protein-effector coupling: a real-time light-scattering assay for transducin-phosphodiesterase interaction.},
  author={Martin Heck and Klaus Peter Hofmann},
  journal={Biochemistry},
  year={1993},
  volume={32 32},
  pages={8220-7}
}
We describe a real-time assay for the interaction of the G-protein of retinal rods, transducin (Gt), with its effector, cGMP phosphodiesterase (PDE). The purified proteins were recombined with isolated rod disk membranes. Reactions were triggered by flash photolysis of the receptor rhodopsin, and monitored by changes of near-infrared light scattering (LS signals). PDE-related LS signals from membrane preparations have been described by others [Caretta, A., & Stein, P. J. (1986) Biochemistry 25… CONTINUE READING