Fyn and ZAP-70 are required for Vav phosphorylation in T cells stimulated by antigen-presenting cells.

@article{Michel1998FynAZ,
  title={Fyn and ZAP-70 are required for Vav phosphorylation in T cells stimulated by antigen-presenting cells.},
  author={Fr{\'e}d{\'e}rique Michel and Linda Grimaud and Loretta Tuosto and Oreste Acuto},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 48},
  pages={31932-8}
}
In T cells, triggering of the T cell antigen receptor or of the co-stimulatory receptor CD28 can direct tyrosine phosphorylation of the signaling protein Vav. We investigated the role played by the protein tyrosine kinases Fyn, Lck, and ZAP-70 in these processes in a T cell hybridoma after physiological stimulation of the T cell receptor (TCR) and CD28. A dominant-negative mutant approach based on overexpression of catalytically inactive alleles of these kinases showed that CD28-induced Vav… CONTINUE READING