Fusion of Lysosomes with Late Endosomes Produces a Hybrid Organelle of Intermediate Density and Is NSF Dependent

@article{Mullock1998FusionOL,
  title={Fusion of Lysosomes with Late Endosomes Produces a Hybrid Organelle of Intermediate Density and Is NSF Dependent },
  author={Barbara M. Mullock and Nicholas A. Bright and Clare W. Fearon and Sally R. Gray and J. Luzio},
  journal={The Journal of Cell Biology},
  year={1998},
  volume={140},
  pages={591 - 601}
}
Using a cell-free content mixing assay containing rat liver endosomes and lysosomes in the presence of pig brain cytosol, we demonstrated that after incubation at 37 degrees C, late endosome-lysosome hybrid organelles were formed, which could be isolated by density gradient centrifugation. ImmunoEM showed that the hybrids contained both an endocytosed marker and a lysosomal enzyme. Formation of the hybrid organelles appeared not to require vesicular transport between late endosomes and… CONTINUE READING
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