Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastids using X-irradiated CMS protoplasts

  title={Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastids using X-irradiated CMS protoplasts},
  author={L{\'a}szl{\'o} Menczel and Alison Morgan and Stacey-Ann Brown and Pal Maliga},
  journal={Plant Cell Reports},
X-irradiated protoplasts of a Brassica napus line carrying the Ogura Raphanus sativus male sterile cytoplasm were fused to protoplasts of male fertile B. napus cv. Olga. Plants were regenerated from six out of 34 randomly selected clones. In one clone, Bn(RS)26, a plant with male sterile flowers was obtained. Mitochondria of this plant are non-parental as revealed by DNA-DNA hybridization using a species specific probe. Its chloroplasts, however, derive from the fertile parent which results in… 
Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion
Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The
Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza safiva L.) protoplasts
Seven of the colonies that formed were identified as cytoplasmic hybrids (cybrids): they all had the peroxidase isozymes of the fertile ‘Fujiminori’ parent, but contained four plasmid-like DNAs from the sterile A-58 CMS parent in their mitochondrial genomes.
Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusion
Cytoplasts isolated from hypocotyl protoplasts of Raphanus sativus cv Kosena by ultracentrifugation through Percoll/mannitol discontinuous gradient were fused with iodoacetamide(IOA)-treated protoplast of Brassica napus c v Westar to show hybridization patterns similar to B. napus.
Transfer of the CMS trait in Daucus carota L. by donor-recipient protoplast fusion
The results indicated that the CMS trait of the donor was efficiently transferred into the cybrid plants by donor-recipient protoplast fusion.
Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production
This chapter retraces the history of using somatic hybridization to modify the cytoplasmic male sterility (CMS)-inducing Ogura radish cy toplasm and its application in hybrid seed production for Brassica crops and details the procedures of the protoplast fusion experiments used to produce cybrids.
A 2.5 kb NcoI fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male-sterility in Brassica cybrids
SummarySpontaneous reversion to fertility was studied in the progeny of a cytoplasmic male-sterile (CMS) Brassica napus cybrid containing recombinant B. napus/Ogura radish mitochondrial genomes. This
Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.)
The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations.
Absence of mitochondrial and chloroplast DNA recombinations in Brassica napus plants regenerated from protoplasts, protoplast fusions and anther culture
SummaryOver 400 Brassica napus plants regenerated from individual protoplasts, from protoplast fusions and from anther culture were analysed for chloroplast and mitochondrial genome rearrangements by
Intergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis
The primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled and it was realised that true somatic hybrids and partial Somatic hybrids had been generated.
Manipulation of Cytoplasmic Genomes
The ability to substitute specific chloroplast populations or plastomes independent of the mitochondrial genome (chondriome) or vice versa is, with a few exceptions, a technique exclusive to the protoplast fusion.


Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant types
A convenient system to assay for genetic recombination between chloroplasts by combining use of several cytoplasmic markers: chlorophyll pigmentation, chloroplast DNA restriction patterns, tentoxin resistance and male sterility is presented.
Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana Sylvestris by fusion with X-irradiated N. tabacum protoplasts
It could be demonstrated that there was an independent segregation of chloroplast type and male fertility/sterility: several somatic cybrids were male fertile but tentoxin sensitive and others were tentoxin insensitive yet they were male sterile.
Cytogenetic analysis of plants regenerated from tissue explants and mesophyll protoplasts of winter rape, Brassica napus L.
Meiotic analysis of regenerated plants showed a range of multiple chromosome associations with no plants consistently exhibiting biva...
Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana.
Chloroplasts of Nicotiana tabacum SR1 were transferred into Nicotiana plumbaginifolia by protoplast fusion. The protoplasts of the organelle donor were irradiated with different lethal doses using a
Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana
SummaryCytoplasmic male sterility (CMS) in Nicotiana is located on mitochondrial DNA (mtDNA) on which no selectable mutation has been isolated. The possibility of co-transfer of CMS and a selectable
Interspecific chloroplast recombination in a Nicotiana somatic hybrid.
In the Pt14 chloroplast genome three N. tabacum and four N. plumbaginifolia parent specific restriction sites have been identified, indicating that the pt14 chloroplasts genome contains at least six recombination sites.
Single Gene Mutations in Tomato Plants Regenerated from Tissue Culture
The recovery of single gene mutations is evidence that plant tissue culture can be mutagenic in Lycopersicon esculentum.
High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae
To obtain a high frequency of regeneration, rapidly growing calli were transferred to media having a high cytokinin:auxin ratio as early as possible, usually 3 weeks after protoplast isolation.