Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastids using X-irradiated CMS protoplasts

@article{Menczel2004FusionmediatedCO,
  title={Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastids using X-irradiated CMS protoplasts},
  author={L{\'a}szl{\'o} Menczel and Alison Morgan and Stacey-Ann Brown and Pal Maliga},
  journal={Plant Cell Reports},
  year={2004},
  volume={6},
  pages={98-101}
}
X-irradiated protoplasts of a Brassica napus line carrying the Ogura Raphanus sativus male sterile cytoplasm were fused to protoplasts of male fertile B. napus cv. Olga. Plants were regenerated from six out of 34 randomly selected clones. In one clone, Bn(RS)26, a plant with male sterile flowers was obtained. Mitochondria of this plant are non-parental as revealed by DNA-DNA hybridization using a species specific probe. Its chloroplasts, however, derive from the fertile parent which results in… 
Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion
Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The
Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza safiva L.) protoplasts
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Seven of the colonies that formed were identified as cytoplasmic hybrids (cybrids): they all had the peroxidase isozymes of the fertile ‘Fujiminori’ parent, but contained four plasmid-like DNAs from the sterile A-58 CMS parent in their mitochondrial genomes.
Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusion
TLDR
Cytoplasts isolated from hypocotyl protoplasts of Raphanus sativus cv Kosena by ultracentrifugation through Percoll/mannitol discontinuous gradient were fused with iodoacetamide(IOA)-treated protoplast of Brassica napus c v Westar to show hybridization patterns similar to B. napus.
Transfer of the CMS trait in Daucus carota L. by donor-recipient protoplast fusion
TLDR
The results indicated that the CMS trait of the donor was efficiently transferred into the cybrid plants by donor-recipient protoplast fusion.
Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production
TLDR
This chapter retraces the history of using somatic hybridization to modify the cytoplasmic male sterility (CMS)-inducing Ogura radish cy toplasm and its application in hybrid seed production for Brassica crops and details the procedures of the protoplast fusion experiments used to produce cybrids.
A 2.5 kb NcoI fragment of Ogura radish mitochondrial DNA is correlated with cytoplasmic male-sterility in Brassica cybrids
SummarySpontaneous reversion to fertility was studied in the progeny of a cytoplasmic male-sterile (CMS) Brassica napus cybrid containing recombinant B. napus/Ogura radish mitochondrial genomes. This
Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.)
TLDR
The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations.
Absence of mitochondrial and chloroplast DNA recombinations in Brassica napus plants regenerated from protoplasts, protoplast fusions and anther culture
SummaryOver 400 Brassica napus plants regenerated from individual protoplasts, from protoplast fusions and from anther culture were analysed for chloroplast and mitochondrial genome rearrangements by
Intergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis
TLDR
The primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled and it was realised that true somatic hybrids and partial Somatic hybrids had been generated.
Manipulation of Cytoplasmic Genomes
TLDR
The ability to substitute specific chloroplast populations or plastomes independent of the mitochondrial genome (chondriome) or vice versa is, with a few exceptions, a technique exclusive to the protoplast fusion.
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