Fusion expression of cecropin B-like antibacterial peptide in Escherichia coli and preparation of its antiserum

Abstract

Antibacterial peptides have a broad range of antibacterial properties that makes them highly toxic for expression in Escherichia coli. For prepare an antiserum to detect these peptides, we developed a cecropin B mutant with a green fluorescent protein fusion partner resulting in high expression of a 37 kDa fusion peptide in E. coli with a yield of 7.9 mg/l… (More)
DOI: 10.1007/s10529-009-0196-x

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