Fusion between endocytic vesicles in a cell-free system.

  title={Fusion between endocytic vesicles in a cell-free system.},
  author={William A. Braell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={84 5},
  • William A. Braell
  • Published 1987 in
    Proceedings of the National Academy of Sciences…
Cell-free fusion between endocytic vesicles has been obtained in a sensitive assay based on the avidin-biotin binding reaction. Chinese hamster ovary cells are allowed to endocytose either avidin-linked beta-galactosidase or biotinylated IgG. Postnuclear supernatant extracts prepared from these cells are incubated at 37 degrees C in the presence of an ATP-regenerating system. Fusion between vesicles from the two extracts permits the avidin-biotin association to occur, so that the amount of… CONTINUE READING
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