Further studies on the site-specific protein modification by microbial transglutaminase.

@article{Sato2001FurtherSO,
  title={Further studies on the site-specific protein modification by microbial transglutaminase.},
  author={Haruya Sato and Eidai Hayashi and Naoyuki Yamada and Mitsuyoshi Yatagai and Yoshifumi Takahara},
  journal={Bioconjugate chemistry},
  year={2001},
  volume={12 5},
  pages={701-10}
}
A guinea pig liver transglutaminase (G-TGase)-mediated procedure for the site-specific modification of chimeric proteins was recently reported. Here, an alternative method with advantages over the recent approach is described. This protocol utilizes a microbial transglutaminase (M-TGase) instead of the G-TGase as the catalyst. M-TGase, which has rather broad structural requirements as compared to the G-TGase, tends to catalyze an acyl transfer reaction between the gamma-carboxamide group of a… CONTINUE READING