Further studies on the binding of divalent cations to the phosphoglycoprotein phosvitin.

@article{Grizzuti1975FurtherSO,
  title={Further studies on the binding of divalent cations to the phosphoglycoprotein phosvitin.},
  author={K{\"a}rt Grizzuti and Gertrude E. Perlmann},
  journal={Biochemistry},
  year={1975},
  volume={14 10},
  pages={
          2171-5
        }
}
Dialysis equilibrium measurements at 25 degrees indicate that, at pH 6.8 and at a concentration of 1.0 times 10(-10) 3 M MnC12 or CoC12, phosvitin binds 113 Mn2+ and 120 Co2+. The binding is cooperative at low cation concentrations. The number of Mg2+, Ca2+, Mn2+, and Co2+ bound is not affected by temperatures of up to 60 degrees; however, the cooperactivity is enhanced. Optical rotatory dispersion and circular dichroism studies indicate that a conformational change occurs on binding of Mn2… CONTINUE READING
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