Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR

  title={Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR},
  author={Fr{\'e}d{\'e}ric Cr{\'e}mazy and Stephan Soullier and Philippe Berta and Philippe Jay},
  journal={FEBS Letters},

HMG-box sequences from microbats homologous to the human SOX30 HMG-box*

Two HMG-box sequences from two microbat species are reported which were PCR amplified using a primer pair specific to the mouse Sry H MG-box and the high percentage of identity of this sequences with the human and mouse SOX30 HMG -box suggests that they are the SOX 30 HMG box for these two bat species.

Isolation of Sox11a, Sox11b and Sox19 genes from Rice field eel (Monopterus albus) using degenerate primers and nested PCR

The two kinds of Sox genes, the Sox11, Sox11a and Sox11b, and the Sox19 genes from rice field eel are presented using highly degenerate and nested PCR.


This work reviews the current knowledge on Sox gene family, their evolution and their functions, and proposes several phylogenetic models for a better understanding of their evolution.

Genome-wide analysis of Sox genes in Drosophila melanogaster

Identification of a novel Sry-related gene and its germ cell-specific expression.

The isolation of a novel member of the Sox gene family, Sox30, is reported, which may constitute a distinct subgroup of this family, and it is shown that it is able to specifically recognize the ACAAT motif.

cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18

The SRY (sex-determining region Y) gene encodes a transcription factor characterized by a DNA-binding motif termed the HMG (high mobility group) domain that is predominantly expressed in heart, although weak signals were seen in brain, liver, testis, and leukocyte.

Chromosome assignments of the rice field eel Sox9 and Sox17 genes

The mammalian Y-linked sex determining gene, SRY (Sex determining region Y), encodes a protein with a DNA-binding domain, HMG box. In a wide variety of animal species, a large family of genes

Isolation and sequencing of the HMG domains of fifteen Sox genes from Hyla sanchiangensis, and analysis of the evolutionary behaviors of Sox duplicated copies based on bioinformatics

It is proposed that all Sox genes in H. sanchiangensis obtained in this examination are under strong purifying selection, and duplicated Sox genes evolved independently.



SOX20, a new member of the SOX gene family, is located on chromosome 17p13.

A data base search revealed a high similarity of the deduced amino acid sequence to the human SOX12 and the murine Sox16 HMG domains, which was assigned to chromosome 17p13 by FISH analysis.

The human SOX11 gene: cloning, chromosomal assignment and tissue expression.

The human SOX11 gene is cloned and characterized using the partial cloning of both human and mouse SoX11 genes and mapped it to chromosome 1p25.

SOX22 is a new member of the SOX gene family, mainly expressed in human nervous tissue.

The cloning of a new human member of the SOX gene family, SOX22, is reported, suggesting that this gene plays roles in both differentiation and maintenance of several cell types.

Partial cloning of SOX-11 and SOX-12, two new human SOX genes.

The amino-acids of the SOX- 1 sequenced so far are identical with Sox-il, a recently described murine homologue and with Lf6 a homolog, and the amino-acid homology with the other human SOX genes very high.

Cloning and characterization of SOX5, a new member of the human SOX gene family.

Cloned SOX5, a new member of the human SOX gene family, shows a high similarity with the mouse Sox5 transcript over a large region identical in all the human cDNAs, however, comparison of the 5' unique sequences of the c DNAs suggests that the SOx5 gene is subject to alternative splicing.

Sox genes find their feet.

A conserved family of genes related to the testis determining gene, SRY.

Sequencing of the cloned PCR products demonstrated that they were heterogeneous, with the clones falling into a few, distinct classes, mostly differing from those SRY-related clones previously described, and the strong evolutionary conservation of this gene family suggests that the SRy-related genes may be of importance in developmental processes.

Ancestry and diversity of the HMG box superfamily.

By studying the extent of diversification of the HMG box superfamily, it is found that the speed of evolution was very different within the various groups of HMG-box containing factors.

A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif

A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene, termed SRY (for sex-determining region Y) and proposed to be a candidate for the elusive testis-d determining gene, TDF.