Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB/ER alpha cells: bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization.

@article{Subramaniam2002FurtherCO,
  title={Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB/ER alpha cells: bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization.},
  author={Malayannan Subramaniam and Syed M. Jalal and David J. Rickard and Steven L. Harris and Mark E. Bolander and Thomas C. Spelsberg},
  journal={Journal of cellular biochemistry},
  year={2002},
  volume={87 1},
  pages={9-15}
}
We have previously generated an immortalized human fetal osteoblastic cell line (hFOB) using stably transfected temperature sensitive SV40 T-antigen (Harris et al. [1995a] J. Bone. Miner. Res. 10:178-1860). To characterize these cells for phenotypic/genotypic attributes desired for a good cell model system, we performed karyotype analysis by multicolor fluorescent in situ hybridization (M-FISH), their ability to form bone in vivo without developing cell transformation, and finally their ability… CONTINUE READING
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