Further characterisation of rotavirus cores: Ss(+)RNAs can be packaged in vitro but packaging lacks sequence specificity☆

@inproceedings{Desselberger2013FurtherCO,
  title={Further characterisation of rotavirus cores: Ss(+)RNAs can be packaged in vitro but packaging lacks sequence specificity☆},
  author={Ulrich Desselberger and James Richards and Luba Tchertanov and Jean Lepault and Andrew M. L. Lever and Oscar R Burrone and Jean Cohen},
  booktitle={Virus research},
  year={2013}
}
Rotavirus (RV) cores were released from double-layered particles (DLPs) by high concentrations of CaCl2, purified and 'opened' by treatment with EDTA or EGTA. Under appropriate in vitro conditions DLPs have been shown to have transcriptase and 'open cores' replicase activity. Furthermore, it has been demonstrated that transcriptase activity and infectivity… CONTINUE READING