Further analyses of epitopes for human monoclonal anti-basement membrane zone antibodies produced by stable human hybridoma cell lines constructed with Epstein-Barr virus transformants.

@article{Hashimoto1993FurtherAO,
  title={Further analyses of epitopes for human monoclonal anti-basement membrane zone antibodies produced by stable human hybridoma cell lines constructed with Epstein-Barr virus transformants.},
  author={Takao Hashimoto and Masayuki Amagai and Tamotsu Ebihara and Shinobu Gamou and Noriaki Shimizu and Takeshi Tsubata and Atsuhiko Hasegawa and Keizaburo Miki and Takeji Nishikawa},
  journal={The Journal of investigative dermatology},
  year={1993},
  volume={100 3},
  pages={310-5}
}
We previously established Epstein-Barr virus (EBV)-transformed bullous pemphigoid (BP) patient lymphoblastoid cell lines, which produced human monoclonal anti-basement membrane zone antibodies. In the present study, we established two independent human-human hybridomas by fusion of these EBV transformants with a human B-cell line. These hybridomas, designated 5E-HY-4B and 10D-HY-8B, were very stable and showed a high yield of monoclonal antibody (MoAb) secretion. Each cell line was tetraploid… CONTINUE READING