Fungal species identification from avian lung specimens by single hypha laser microdissection and PCR product sequencing.

@article{Olias2011FungalSI,
  title={Fungal species identification from avian lung specimens by single hypha laser microdissection and PCR product sequencing.},
  author={Philipp Olias and Ilse D Jacobsen and Achim D Gruber},
  journal={Medical mycology},
  year={2011},
  volume={49 1},
  pages={56-61}
}
Accurate species diagnosis in cases of fungal pneumonia may be hampered by environmental contamination and colonization resulting in false-positive results. Our novel approach for fungal species diagnostics combines fluorescent staining of mounted cryosections with the optical brightener Blankophor, laser capture microdissection and PCR amplification with subsequent sequencing of the first internal transcribed spacer region (ITS-1). Using clinical specimens from infected birds, we show that the… CONTINUE READING