• Corpus ID: 5658182

Fungal molecular diagnostics: a mini review.

@article{Atkins2004FungalMD,
  title={Fungal molecular diagnostics: a mini review.},
  author={Simon D. Atkins and Ian M. Clark},
  journal={Journal of applied genetics},
  year={2004},
  volume={45 1},
  pages={
          3-15
        }
}
Conventional methods to identify fungi have often relied on identification of disease symptoms, isolation and culturing of environmental organisms, and laboratory identification by morphology and biochemical tests. Although these methods are still fundamental there is an increasing move towards molecular diagnostics of fungi in all fields. In this review, some of the molecular approaches to fungal diagnostics based on polymerase chain reaction (PCR) and DNA/RNA probe technology are discussed… 
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References

SHOWING 1-10 OF 46 REFERENCES
Molecular diagnostics for fungal plant pathogens.
TLDR
PCR-based methods can provide new tools to monitor the exposure of a crop to pathogen inoculum that are more reliable and faster than conventional methods that can be used to improve disease control decision making.
Rapid detection and diagnosis of Septoria tritici epidemics in wheat using a polymerase chain reaction/PicoGreen assay
TLDR
The PCR/PicoGreen assay was applied successfully to study the colonization, infection and subsequent disease development of S. tritici on wheat, both under controlled conditions in the glasshouse and in the field.
Conventional PCR and Real-time Quantitative PCR Detection of Helminthosporium Solani in Soil and on Potato Tubers
TLDR
This rapid and quantitative PCR assay allows an accurate estimation of tuber and soil contamination by H. solani, thus providing a tool to study the ecology of the organism and to serve as a crucial component for disease risk assessments.
Approaches for monitoring the release of Pochonia chlamydosporia var. catenulata, a biocontrol agent of root-knot nematodes.
TLDR
The development of a baiting method allowed quick in situ screening of the isolates' ability to infect nematode eggs, and when combined with PCR diagnosis both varieties of the fungus could be detected in infected eggs.
The application of real-time PCR to the identification, detection and quantification of Pyrenophora species in barley seed.
Summary A real-time quantitative PCR technique has been used to develop a rapid and sensitive seed health test for Pyrenophora spp. on barley seed. Using the fluorescent reporter dye SYBR Green I for…
PCR-based molecular discrimination of Verticillium chlamydosporium isolates
Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction.
TLDR
Real-time PCR is the only method currently available to quantify P. gaeumannii colonization early in the first year of the colonization process, and eliminated the need to analyze samples in multiple dilutions when comparing lightly with heavily infected needles.
Development of PCR for the detection of Polymyxa betae in sugar beet roots and its application in field studies
TLDR
PCR was used to detect P. betae in a survey of infection incidence in 65 sugar beet fields, with a lower incidence on peat as compared to mineral soils and protocols which combine PCR with Southern/dot blot analysis of products were developed.
Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in…
Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants
Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify…
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