Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea

  title={Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea},
  author={Ernesto P. Benito and Arjen ten Have and John W. van 't Klooster and Jan A. L. van Kan},
  journal={European Journal of Plant Pathology},
An inoculation procedure was developed to obtain efficient and synchronous infection on detached tomato leaves by Botrytis cinerea. In spray-inoculated leaves incubated at 20 °C, the infection process consisted of three phases: the formation of primary necrotic lesions (until 20 hpi), a quiescent phase (20-72 hpi), and the expansion of a proportion of the primary lesions (from 72 hpi onwards), resulting in full tissue maceration. At 4 °C, the infection progressed slowly but steadily without… CONTINUE READING
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