Fungal Adenylyl Cyclase Integrates CO2 Sensing with cAMP Signaling and Virulence

@article{Klengel2005FungalAC,
  title={Fungal Adenylyl Cyclase Integrates CO2 Sensing with cAMP Signaling and Virulence},
  author={Torsten Klengel and Wei-jun Liang and James Chaloupka and Claudia Ruoff and Klaus Schröppel and Julian R. Naglik and Sabine E. Eckert and Estelle Gewiss Mogensen and Ken Haynes and Mick F. Tuite and Lonny R. Levin and Jochen Buck and Fritz A. Mühlschlegel},
  journal={Current Biology},
  year={2005},
  volume={15}
}
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TLDR
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CO2 Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
TLDR
The results reveal the existence of a gaseous Candida signalling pathway and its molecular mechanism and provide insights into an evolutionary conserved CO2-signalling system, suggesting that metabolic CO2 sensing may be important for initial colonisation and epithelial invasion.
CO 2 Acts as a Signalling Molecule in Populations of the Fungal Pathogen Candida albicans
TLDR
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References

SHOWING 1-10 OF 38 REFERENCES
Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans.
TLDR
It is proposed that CaCdc35p acts downstream of the Ras homologue CaRas1p and components of the hyphal-inducing MAP kinase pathway depend on the function of CaCDC35p in their ability to induce morphogenetic switching.
Adenylyl Cyclase Functions Downstream of the Gα Protein Gpa1 and Controls Mating and Pathogenicity of Cryptococcus neoformans
TLDR
Findings provide direct evidence that Gpa1 and adenylyl cyclase function in a conserved signal transduction pathway controlling cAMP production, hyphal differentiation, and virulence of this human fungal pathogen.
Ras links cellular morphogenesis to virulence by regulation of the MAP kinase and cAMP signalling pathways in the pathogenic fungus Candida albicans
TLDR
Results indicate that, in response to environmental cues, CaRas1p is required for the regulation of both a MAP kinase signalling pathway and a cAMP signalling pathway, and contributes to the pathogenicity of C. albicans cells through the induction of polarized morphogenesis.
A Defined Subset of Adenylyl Cyclases Is Regulated by Bicarbonate Ion*
TLDR
This work proposes that Lys-646 specifically coordinates bicarbonate in the active site in conjunction with an aspartate to threonine polymorphism conserved in class III adenylyl cyclases from diverse eukaryotes and prokaryotes, which represents a novel mechanism by which diverse organisms can detect bICarbonate ion.
Virulence of Cryptococcus neoformans. Regulation of capsule synthesis by carbon dioxide.
TLDR
The prevailing CO2 concentration in mammalian tissues may be one stimulus for capsular polysaccharide synthesis in Cryptococcus neoformans, which could serve as an adaptive mechanism favoring parasite survival in the host.
Ras Signaling Is Required for Serum-Induced Hyphal Differentiation in Candida albicans
TLDR
Low-molecular-weight molecules in serum induce hyphal differentiation in C. albicans through a Ras-mediated signal transduction pathway, and strains expressing the dominant active Ras1(V13) allele manifest enhanced hyphal growth, whereas those expressing a dominant negative RAS1(A16) allele show reduced hyphalgrowth.
The pH of the Host Niche Controls Gene Expression in and Virulence of Candida albicans
TLDR
The results suggest that the pH of the infection site regulates the expression of genes essential to survival within that niche, and implies that the study of environmentally regulated genes may provide a rationale for understanding the pathobiology of C. albicans.
Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor.
TLDR
It is demonstrated here that bicarbonate directly stimulates mammalian soluble adenylyl cyclase (sAC) activity in vivo and in vitro in a pH-independent manner, which suggests that bICarbonate regulation of cAMP signaling plays a fundamental role in many biological systems.
Aquaporin in Candida: characterization of a functional water channel protein
TLDR
Deletion of AQY1 in C. albicans resulted in cells that were less sensitive than wild‐type to osmotic shock and there was no difference between the virulence of C.Albicans wild‐ type and aqy1 null strains in a murine model for systemic candidiasis.
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