[Fundamental studies on differentiation inducers using a human embryonal carcinoma model].


A human embryonal carcinoma (EC) cell line, NEC14, was pluripotent and the tumors formed in nude mice contained differentiated somatic and extra-embryonic elements in addition to EC. The NEC14 cells could be induced to differentiate with 5 differentiation inducers in vitro, and N,N'-hexamethylene bisacetamide (HMBA) showed the most potent differentiator induction among them. All of the NEC14 cells were morphologically differentiated when exposed to 10(-2) M HMBA for 3 days. The differentiated derivatives ceased to proliferate in vitro and became non-tumorigenic in nude mouse. Several differentiation markers appeared in the differentiated derivatives. When the tumor-bearing nude mice were injected intraperitoneally with HMBA (10mg, twice a week), the tumor growth was inhibited. These results show that human EC is a useful model of differentiation-induction in cancer therapy.

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@article{Yamazawa1991FundamentalSO, title={[Fundamental studies on differentiation inducers using a human embryonal carcinoma model].}, author={Koji Yamazawa and Souei Sekiya and Hiroshi Kimura and Hiroshi Shirasawa and Mitsuhiro Kawata and Hiroyoshi Takamizawa}, journal={Nihon Sanka Fujinka Gakkai zasshi}, year={1991}, volume={43 10}, pages={1303-9} }