Functions of purified E1A protein microinjected into mammalian cells.

  title={Functions of purified E1A protein microinjected into mammalian cells.},
  author={Bernd Krippl and Betsy Ferguson and Martin Goldstern Hajime Machida Ivo G. Rosenberg and Heiner Westphal},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={81 22},
We have purified the human type C adenovirus E1A 13S mRNA gene product, expressed in Escherichia coli, and demonstrate that the protein exhibits genuine viral functions upon microinjection into mammalian cells. We show that the E1A protein activates expression of the adenovirus E2A gene and induces expression from the major late transcription unit of the adenovirus E1A deletion mutant, H5dl312. We use this functional assay to examine the stability of E1A protein microinjected into cells and… CONTINUE READING