Functions of crystallins in and out of lens: roles in elongated and post-mitotic cells.

@article{Slingsby2014FunctionsOC,
  title={Functions of crystallins in and out of lens: roles in elongated and post-mitotic cells.},
  author={C. Slingsby and G. Wistow},
  journal={Progress in biophysics and molecular biology},
  year={2014},
  volume={115 1},
  pages={
          52-67
        }
}
The vertebrate lens evolved to collect light and focus it onto the retina. In development, the lens grows through massive elongation of epithelial cells possibly recapitulating the evolutionary origins of the lens. The refractive index of the lens is largely dependent on high concentrations of soluble proteins called crystallins. All vertebrate lenses share a common set of crystallins from two superfamilies (although other lineage specific crystallins exist). The α-crystallins are small heat… Expand
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References

SHOWING 1-10 OF 190 REFERENCES
Evolution of crystallins for a role in the vertebrate eye lens
TLDR
The camera eye lens of vertebrates is a classic example of the re‐engineering of existing protein components to fashion a new device and the structural and genetic features of these two protein superfamilies that enable the rapid creation of different assembly states are shown to match the rapidly changing optical needs among the various vertebrates. Expand
Crystallins in the eye: Function and pathology
  • U. Andley
  • Medicine, Biology
  • Progress in Retinal and Eye Research
  • 2007
TLDR
Recent work with lens epithelial cells from alphaA- and alphaB-crystallin knockout mice suggests that crystallins have important cellular functions in the lens epithelium and not just the lens fiber cells as previously thought. Expand
Structure and dynamics of the fish eye lens protein, γM7-crystallin.
TLDR
NMR relaxation data show increased backbone conformational motions in the loop regions of γM7 compared to those of mouse γS- Crystallin and show that fast internal motion of the interdomain linker in γ-crystallins correlates with linker length. Expand
Crystallins in Retinal Ganglion Cell Survival and Regeneration
TLDR
The findings demonstrate the importance of crystallin genes in RGC survival and regeneration and further in-depth studies are necessary to better understand the mechanisms underlying the functions of these proteins in healthy RGCs as well as during glaucomatous neurodegeneration, which in turn could help in designing new therapeutic strategies to preserve or regenerate these cells. Expand
Novel roles for α-crystallins in retinal function and disease
TLDR
Generation of liposomal particles and other modes of nanoencapsulation of these minipeptides could offer great therapeutic advantage in ocular delivery for a wide variety of retinal degenerative, inflammatory and vascular diseases including age-related macular degeneration and diabetic retinopathy. Expand
Ageing and vision: structure, stability and function of lens crystallins.
TLDR
The structure, assembly, interactions, stability and post-translational modifications of the crystallins are reviewed, not only in isolation but also as part of a multi-component system. Expand
Urochordate βγ-Crystallin and the Evolutionary Origin of the Vertebrate Eye Lens
TLDR
The conservation of the regulatory hierarchy controlling βγ-crystallin expression between organisms with and without a lens shows that the evolutionary origin of the lens was based on co-option of pre-existing regulatory circuits controlling the expression of a key structural gene in a primitive light-sensing system. Expand
A role for γS‐crystallin in the organization of actin and fiber cell maturation in the mouse lens
TLDR
In’vitro experiments showed that recombinant mouse γS can directly stabilize F‐actin, suggesting that δS may have a functional role related to actin, perhaps in ‘shepherding’ filaments to maintain the optical properties of the lens cytoplasm and normal fiber cell maturation. Expand
The molecular refractive function of lens γ-Crystallins.
TLDR
It is shown that all lens γ-Crystallins have evolved a significantly elevated molecular refractive index increment, which is far above those of most proteins, including nonlens members of the βγ-crystallin family from different species. Expand
The presence of extralenticular crystallins and its relationship with transdifferentiation to lens.
TLDR
This chapter reviews recent studies on the extralenticular distribution of crystallins and discusses its possible role in the transdifferentiation of nonlenticular tissues into lens. Expand
...
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3
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5
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