Functions of a rho-specific guanine nucleotide exchange factor in neurite retraction. Possible role of a proline-rich motif of KIAA0380 in localization.

@article{Togashi2000FunctionsOA,
  title={Functions of a rho-specific guanine nucleotide exchange factor in neurite retraction. Possible role of a proline-rich motif of KIAA0380 in localization.},
  author={Hideo Togashi and Koji Nagata and Mihoko Takagishi and Noriko Saitoh and Masaki Inagaki},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 38},
  pages={29570-8}
}
The Rho/Rho kinase signaling pathway plays an essential role in neurite retraction and cell rounding in response to G(12/13)-coupled receptor activation in neuronal cells. The Rho guanine nucleotide exchange factor involved in these processes has not been identified. To monitor the activation state of Rho kinase, we developed a vimentin head/Rho kinase chimera, which is intramolecularly phosphorylated in a Rho-dependent manner at Ser(71) of the fused vimentin head. Using this system, we… CONTINUE READING